Median Lethal Dose of Aeromonas salmonicida on Amago Salmon (Oncorhynchus rhodurus)

Abstract

Median lethal doses (LD₅₀) of Aeromonas salmonicida on Amago salmon, Oncorhynchus rhodurus were studied by three different infection routes.
LD₅₀ of the bacteria on Amago salmon by intramuscular injection, intraperitoneal injection and bathing method (water-borne injection) were estimated as 4.3-5.1 × 10⁰ CFU/fish, 3.0-4.5 × 10³ CFU/fish and 2.2-2.7 × 10⁶ CFU/ml, respectively.
The fluctuations of LD₅₀ obtained in the each duplicate experiments were ignorable. This suggests the advantage of using genetic isolated stocks of fish for the experimental animal.