Abstract
High agglutinating antibody titer serum of colored carp Cyprirus carpio was obtained after 4 times injection of formalin-killed Aeromonas hydrophila cells. The immunoglobulin of immunized carp was purified by gel filtration on Sepharose 6B and ion exchange chromatography on DEAE-Sephadex A-25.
Agglutinating antibody activity of immunized carp was found in the second fraction using Sepharose 6B and DEAE-Sephadex A-25. The protein concentration of immunized carp was 39.84 mg/ml and the immunoglobulin accounts for about 5.5 % of the serum protein. The treatment of immunoglobulin with 2-ME reduced completely the agglutinating antibody activity.
From the results of this study it is suggested that the immunoglobulin of colored carp resembles that of the IgM type in mammals.
