Identification of a Novel Chromosomal Region Associated with Infectious Hematopoietic Necrosis (IHN) Resistance in Rainbow TroutOncorhynchus mykiss

Sok Kean Khoo; Akiyuki Ozaki; Fusayuki Nakamura; Tetsuya Arakawa; Shinichi Ishimoto; Radoslav Nickolov; Takashi Sakamoto; Tetsuya Akutsu; Mamiko Mochizuki; Ikuo Denda; Nobuaki Okamoto

doi:10.3147/jsfp.39.95

Sok Kean Khoo, Akiyuki Ozaki, Fusayuki Nakamura, Tetsuya Arakawa, Shinichi Ishimoto, Radoslav Nickolov, Takashi Sakamoto, Tetsuya Akutsu, Mamiko Mochizuki, Ikuo Denda, Nobuaki Okamoto

Author information

Sok Kean Khoo
Laboratory of Cancer Genetics, Van Andel Institute
Department of Marine Bioscience, Tokyo University of Marine Science and Technology
Akiyuki Ozaki
Department of Marine Bioscience, Tokyo University of Marine Science and Technology
Department of Zoology, University of Guelph
Fusayuki Nakamura
Mikawaichinomiya Station, Freshwater Resources Research Center, Aichi Fisheries Research Institute
Tetsuya Arakawa
Mikawaichinomiya Station, Freshwater Resources Research Center, Aichi Fisheries Research Institute
Shinichi Ishimoto
Mikawaichinomiya Station, Freshwater Resources Research Center, Aichi Fisheries Research Institute
Radoslav Nickolov
Department of Mathematical Sciences, Michigan Technological University
Takashi Sakamoto
Department of Marine Bioscience, Tokyo University of Marine Science and Technology
Tetsuya Akutsu
Fuji Trout Hatchery, Shizuoka Prefectural Fisheries Experimental Station
Mamiko Mochizuki
Fuji Trout Hatchery, Shizuoka Prefectural Fisheries Experimental Station
Ikuo Denda
Nagano Prefectural Fisheries Experimental Station
Nobuaki Okamoto
Department of Marine Bioscience, Tokyo University of Marine Science and Technology

Keywords: infectious hematopoietic necrosis, IHN resistance, genetic linkage map, microsatellite marker, chromosomal region, Oncorhynchus mykiss, rainbow trout

JOURNAL FREE ACCESS

2004 Volume 39 Issue 2 Pages 95-101

DOI https://doi.org/10.3147/jsfp.39.95

Details

Abstract

Infectious hematopoietic necrosis (IHN) is an acute, systemic, rhabdoviral disease of salmonid species. To date, the effective approach in preventing or treating this epizootic disease is unknown. In this study, a genetic cross was established to elucidate the genetic markers associated with IHN resistance in rainbow trout Oncorhynchus mykiss. An IHN-resistant paternal strain of was crossed to a susceptible maternal strain to produce a F₁generation. Mating F₁males back to the maternal strain created F₂, a backcross generation. Five backcross families were generated and IHNV was challenged on 92 individuals of each family. Linkage analysis was performed on the backcross families using 96 microsatellite markers from 25 linkage groups. LOD score as well as p values using the Pearson goodness of χ²-test were calculated to identify the chromosomal regions associated with IHN resistance. The highest LOD score of 0.952 (θ=0.39) was obtained in one backcross family. A novel chromosomal region (p = 0.011) on linkage group 2 consists of a cluster of 4 microsatellite markers, OmyFGT3TUF, OmyFGT25TUF, OmyRGT35TUF and BFRO0001, and they were identified as a potential region for quantitative trait loci of IHN resistance in rainbow trout.

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