Abstract
Two Japanese isolates of viral hemorrhagic septicemia virus (VHSV), JF00Ehi1 (Genogroup I) and KRRV-9601 (Genogroup III), from Japanese flounder Paralichthys olivaceus were examined for the virulence to rainbow trout Oncorhynchus mykiss and 4 species of marine fish. Rainbow trout injected intraperitoneally with each isolate at a dose of 107.5 TCID50/fish showed neither death nor hemorrhage in the skeletal muscle, while the mortalities in Japanese flounder were both 100%. The mortalities by intramuscular injection with JF00Ehi1 at a dose of 107.0 TCID50/fish were 95.0%, 75.0% and 97.5% in black rockfish Sebastes inermis, red sea bream Pagrus major and yellowtail Seriola quinqueradiata, respectively.
