Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
Studies on the Pathogenic Myxobacteria-I
Isolation, Culture and artificial Infection
Kishio HATAIToshikazu HOSHINA
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1971 Volume 5 Issue 2 Pages 100-106

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Abstract

In the summer from 1967 to 1969, 45 strains of Chondrococcus columnaris were isolated from 36 individuals of fishes suffering from columnaris disease which include eels (Anguilla japonica), rainbow trout (Salmo gairdneri) and carp (Cyprinus carpio).
The majority of the bacteria was isolated from the gill, but some of them were from internal organs (kidney, liver and heart). This fact suggests that the bacteria may invade in such internal organs. However, juding from isolation of bacteria on the agar plates, microscopical examination of smear preparations from and histological examination, of the internal organs it seems that the bacteria can not multiply in the internal organs. Therefore, it appears that the pathogenic function of the bacteria can come into play only on the gill and body surface which are under the aerobic environmental conditions.
Four strains isolated from eels, and two strains each isolated from carp and rainbow trout were selected from the stock strains for artificial injection experiments with loaches (Misgurnus anguillicaudatus). The result was that two of the strains (isolated in the summer of 1967) which had been maintained for a year could not cause the disease to the loaches. The succeeding experiment was performed by the other four strains isolated in the summer of 1968. Experimental fishes were dipped in a dilute suspension of bacteria or were smeared with the bacteria on lesions made by an artificial burn, and were kept in water-flowing aquaria. The water temperature was 25 to 28°C. The result was that the loaches showed the obvious symptoms of columnaris disease after 24 to 72 hours, and died.

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© The Japanese Society of Fish Pathology
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