Abstract
Drug levels in yellowtail(Seriola quinqueradiate)tissues following a single administration of chloramphenicol were studied. The fish used were 105-185 gr. in body weight. The test was done at about 27°C. Samples of fish were used.for analysis at each sampling.
The fish divided into two groups, about 6 hours after administration. One group was given a diet after administration(nonfasting group)and another group (fasting group) recieved no feed during the course of experiment. Sampling for analysis was done at 2, 6, 24, 48, 72, and 120 hours after administration.
Following a single feeding at 50 mg CP/Kg fish, yellowtail maintained levels(μg drug/gr. or ml tissue)of 16, 5-20.6, 14.4-18.6, 6.2-7.8, 6.2-6.8 and 4.5-5.0 in liver, spleen, blood, kidney and muscle, respectively, between 2-6 hours after administration.
In the fasting group, 24 hours after administration liver, spleen and kidney still contained appreciable amount of CP and no trace of the drug were detected in muscle and blood. The drug was not detectable in kidney, liver and spleen after 48, 72 and 120 hours after admistration.
On the contrary the drug concentrations in the tissues tended to decrease somewhat faster in nonfasting fish than in fasting fish. No traces of the drug were detected in kidney, blood and muscle, and liver and spleen after 24, 48 and 72 hours after administration respectively.
Total nitro compound levels in liver and muscle tended to be a little higher in nonfasting fish than in fasting fish. This suggested the possibility that the diet used might contain some factors which responsed to Tsuda's reagent.
The duration of retention of drug in the tissues was shorter in this experiment done at 27°C than that at 22°C reported previously.
