A large volume of lubrication oil is used in factories performing metal cutting work. However, water-soluble oil decays due to bacterial breeding, and the resulting smell is problematic in the working environment. Drugs using microorganisms are available to prevent this bacteria-induced decay, but the effects and mechanisms have yet to be examined. So, in this study, the sterilization and growth-inhibiting effect of Biological-material-solution (BMS) on putrefactive bacteria were investigated. The characteristics of BMS, especially its antioxidant activity, were confirmed. In addition, the relationships were considered.
Antioxidant activity was shown by BMS. The activity weakened when BMS was filtrated. A weak sterilizing effect was also shown by BMS. However, it was insufficient to control putrefaction and the putrid odor. Further, there was no association between the antioxidant activity and sterilization effects. The antioxidant actions are therefore not the main factors which control putrefaction of a water-soluble oil. The dose needed to inhibit a certain number of putrefactive bacteria was associated with the quantity of BMS added.
In conclusion, BMS suppressed the activity of putrefactive bacteria, and did not exhibit bactericidal activity. This suppressant activity was dependent on the substrate metabolized from BMS. In this study, it was necessary to remove live microorganisms from BMS in the examination method. So, when live microorganisms exist, the suppressant effect will strengthen, or the effective time will become longer. Actual on-site examination is necessary for verification, and a future study is anticipated.
