In this study, the 2-years changes in bone mineral density (BMD) and lifestyle for Japanese women in thirties, 127 in number, were evaluated to investigate the influence of lifestyle on BMD. The major results were as follows. 1. The subject women whose bone density was higher tended to have experienced menarche in early ages and physical exercise more frequently. 2. Two thirds of the subject women reduced BMD during 2 years, although the women having lower BMD at the 1st-year measurement showed lower reduction of BMD and increased exercise and intake of Ca-contained medicines. 3. The women who increased BMD during 2 years took more calcium-rich foods, e.g. "milk and dairy product" hand "green and yellow vegetables". 4. The health examination and service about BMD was useful to improve lifestyle for women whose BMD was lower. These findings evidence changeable BMD of women in thirties according to lifestyle against the previous supposition that it was scarcely changed. Good nutritional status caused by adequate calcium intake in particular triggers increase of BMD. In this concern, health examination and service about BMD is beneficial to women for increasing awareness of their BMD and motivation of changing their lifestyle.