Abstract
High-pressure fluorescence study on the bilayer phase behavior of symmetric and asymmetric phospholipids in my doctoral thesis is briefly reviewed. It has been clarified by the novel analysis of the second derivatives of fluorescent spectra that the fluorescent probe Prodan resides multiple sites in the bilayer membrane of phosphatidylcholines (PCs) depending on the membrane states. Comparing the diagrams of three dimensional fluorescent spectra among them, the present results revealed that the slight difference in acyl chain length between the sn-1 and sn-2 positions in the PC molecules provides significant influence on their bilayer phase behavior, especially in the bilayer interdigitation.
