2015 年 25 巻 4 号 p. 334-342
We found the feasibility of sterilization by applying a pressure treatment of 200 MPa before heat treatment. The wet density of spores of Bacillus cereus was decreased by the pressure history when we applied a high-pressure treatment of 200 MPa, and the heat resistance of the spores was decreased. Afterwards when we applied a heat at a medium-temperature of about 100℃, the sterilization of spores was promoted. Furthermore, we found the turbidity of the spore suspensions greatly decreased after the pressure reduction step of the high-pressure treatment. The decrease of turbidity was also found to be highly correlated with the survival spore counts after the heat treatment. Therefore the turbidity was considered to be a sterilization indicator.