Abstract
In bone marrow, stromal cells may provide the microenvironment for self-renewal and differentiation of hematopoieticstem/progenitor cells through complex cell-cell interaction. To elucidate the regulatory mechanisms ofhematopoiesis by stromal cells, we established many stromal cell lines from various hematopoietic organs. Wethen reconstructed the in vitro hematopoietic microenvironment and succeeded to establish the stroma-dependenthematopoietic cell lines. This in vitro system may be useful to find regulatory factors involved in the hematopoietic microenvironment.
