A method for gene transfer, single isolation and in vitro assay for neural stem cells

Masanori Sakaguchi; Kazunobu Sawamoto; Takuya Shimazaki; Toshio Kitamura; Akira Shibuya; Hideyuki Okano

doi:10.2492/jsir.25.50

Original Article

A method for gene transfer, single isolation and in vitro assay for neural stem cells

Masanori Sakaguchi, Kazunobu Sawamoto, Takuya Shimazaki, Toshio Kitamura, Akira Shibuya, Hideyuki Okano

著者情報

キーワード: FACS, functional assay, neural stem cell, retrovirus, self-renew

ジャーナルフリー

2005 年 25 巻 1 号 p. 50-54

DOI https://doi.org/10.2492/jsir.25.50

詳細

抄録

The neurosphere method is widely used to examine self-renewal activity and the multipotency of neural stem cells (NSCs). We developed an efficient method for introducing a transgene into neurospheres using a retroviral vector and isolating a transgenic subpopulation of NSCs labeled with green fluorescent protein (GFP) using fluorescence-activated cell sorting, enabling these cells to be used in subsequent biological assays. This method is expected to be useful for studying the function of genes in NSCs.

責任著者(Corresponding author)

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