Flow cytometric studies of oxydative products in neutrophile

—influence of time and temperature from sampling to assay in collagen disease—

Abstract

By flow cytometric method with dichlorofluorescin diacetate, we analysed oxidative products of neutrophils in patients with Behcet disease, systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and progressive systemic sclerosis (PSS) .
The rate of “primed” cells that produced oxidative products in unstimulated neutrophils was higher in patients than in normal control, but in PMA-stimulated neutrophils, there was no differences in patients and normal control. And there were statistical correlations of the primed cells in unstimulated neutrophils and some markers of disease acitivities. This flow cytometric method could clarify the influence of time and temperature from sampling to assay, and was useful to study the functions of cells.