Abstract
We have isolated a cytotoxic factor from the submandibular glands of male and female BALB/c mice using Sephadex G-50 gel filtration chromatography and reverse-phase HPLC. SDS-PAGE and amino acid sequence analysis revealed that the cytotoxic factor was mouse glandular kallikrein (mGK) . The cytotoxic factor was the mixture of mGK-6 and -9 in male mice and mGK-6 in female mice.
The purified mGK showed a cytotoxicity against mouse thymocytes in a dose-dependent manner and a enzyme activity of tissue kallikrein.
