Abstract
Employing interferon gamma activated human monocyte cell line, THP-1, we have demonstrated that luminol dependent chemiluminescence (CL) and lucigenin dependent CL are simultaneously elicited by phagocytosis of murine IgG2a coated SRBC, an immune complex that specifically binds to FcR I on THP-1. Conversely, only lucigenin dependent CL and no luminol dependent CL has been elicited by unphagocytosable FcR I stimulus. The results suggest that NADPH oxidase system and myeloperoxidase-halide system are triggered upon stimulation of a single class of IgG Fc receptor, and that the former system is activated merely upon FcR I stimulation and the latter upon phagocytosis mediated by the same receptor.
