炎症
Online ISSN : 1884-4006
Print ISSN : 0389-4290
ISSN-L : 0389-4290
カテプシンGのリンパ球活性化作用
長谷 聖美青木 洋祐
著者情報
ジャーナル フリー

1991 年 11 巻 5 号 p. 441-445

詳細
抄録
Cathepsin G is one of serine proteases with chymotrypsin-like proteolytic activity and known to exist in neutrophils and spleens. We purified the protease from human bone marrow cells by extraction with 1 M potassium phosphate buffer (pH 7.0) and ap-plying to column chromatography of DEAF-cellulose, Sephadex G-100, CM-cellulose, and Mono-S. We further separated it into three isozymes by employing hydrophobic chromatography, and named them cathepsin.
Purified cathepsin G potentiated the effect of mitogens such as Con A and PHA on human lymphocytes. The protease further stimulated synthesis of both DNA and RNA of lymphocytes in the absence of mitogens. Effect of cathepsin G on the lymphocyte activity was prevented by inhibitors of cathepsin G. Therefore, the effect of it on lymphocyte activity is considered to be due to its proteolytic activity. The effect of cathepsin G on the function of lymphocytes differed among isozymes of the protease. From these results, we suppose that cathepsin G plays an important role in biophylaxis by regulating functions of lymphocytes.
著者関連情報
© 日本炎症・再生医学会
前の記事 次の記事
feedback
Top