抄録
We examined the effects of histamine on interle-ukin-6 (IL-6) production in human peripheral blood lymphocytes (PBL) .
PBL obtained from healthy volunteers were cultured in RPMI 1640 with 1% FCS for 72 hours. The effects of histamine, 2-pyridylethylamine (H1 receptor agonist) and dimaprit (H2 receptor agonist) on phyto-hemagglutinin (PHA) -driven IL-6 production and intra-cellular cyclic AMP (cAMP) accumulation were analyzed with time course.
The production of IL-6 in PBL was gradually increased by 24 hours with reaching plateau after PHA activation. Both histamine and dimaprit significantly reduced the production of IL-6 in PBL, but 2-pyridylethylamine did not. The content of intra-cellular cAMP in PBL was rapidly increased after incubation with both histamine and dimaprit.
These results indicated that histamine increased intra-cellular cAMP and then reduced IL-6 production in PBL through H2 receptor.
