Abstract
The effect of several agents on mitogen-induced proliferative responsein vitrowas studied by using colorimetric MTT [3- (4, 5-dimenthyl-2-thiazolyl) -2, 5-diphenyl-2H tetrazolium bromide] assay. Immuno-modulator levamisole and TOK-8801 enhanced concanavalin A and lipopolysaccharide response in murine splenocytes at concentrations of 10-6-10-5M and 10-4M, respectively. Immunosuppressant mizoribine decreased dose dependently this response.
These results show that MTT assay may be a useful method for the evaluation of immunological affecting agents on cell proliferative response.
