Abstract
Anti-inflammatory effects of TSA-234 (recombinant human superoxide dismutase, rhSOD) were studied in vitroand in vivo. TSA-234, 0.1-100 μg/ ml, significantly inhibited damage of chondrocytes, reduction inviscosity of hyaluronic acid and osteoarthrosis-derived joint fluid, delayed gelation of collagen and inactivation of α1-proteinase inhibitor (PI) which were induced by active oxygen species generated in the system of hypoxanthine-xanthine oxidase. Intraarticular injection of TSA-234 significantly inhibited both carrageenin-induced arthritis in rabbit knees and sodium urate-induced arthritis in dog knees. Furthermore, TSA-234 significantly inhibited rat carrageenin-induced paw edema and mouse ischemic paw edema by 0.125-1 mg/kg, i. v. and 1 mg/kg, i. v., respectively.
These results suggest that TSA-234 will be useful with an Intraarticular injection on the therapy of osteoarthrosis in knees.
