実験腎炎における糸球体マトリックスメタロプロテイネース活性の変動

服部 智久; 進藤 省一郎; 藤塚 直樹; 日比野 智子; 寺園 芳樹; 古泉 素明; 黒木 亮子; 佐藤 俊次; 遠藤 徹; 丸野 政雄

doi:10.2492/jsir1981.15.467

Changes in matrix metalloproteinase activities in glomeruli of experimental glomerulonephritis

Tomohisa Hattori, Shoichiro Shindo, Naoki Fujitsuka, Tomoko Hibino, [in Japanese], Motoaki Koizumi, Akiko Kurogi, Toshitsugu Sato, Toru Endo, Masao Maruno

Author information

Tomohisa Hattori
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
Shoichiro Shindo
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
Naoki Fujitsuka
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
Tomoko Hibino
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
[in Japanese]
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
Motoaki Koizumi
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
Akiko Kurogi
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
Toshitsugu Sato
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
Toru Endo
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.
Masao Maruno
New Drug Research Department, Institute of New Drug Research, Tsumura Central Laboratories, Tsumura & Co.

Keywords: GBM, nephritis, metalloproteinase, Thy-1, TIMP

JOURNAL FREE ACCESS

1995 Volume 15 Issue 6 Pages 467-474

DOI https://doi.org/10.2492/jsir1981.15.467

Details

Abstract

The purpose of this study was to investigate the changes in matrix accumulation, matrix metalloproteinase (MMP) activities and tissue inhibitor of metalloproteinase (TIMP) expression in glomeruli of anti-GBM and anti-Thy-1 nephritic rats. In glomeruli of anti-GBM nephritic rats, an increase in fibrous crescent was observed from day 20 to day 35. The activities of MMP 1, 2 and 9 significantly decreased in glomeruli of anti-GBM nephritic rats from day 1 to day 35. TIMP expression in mesangial area of anti-GBM nephritic rats increased rapidly from the 1st day with a peak of day 10. In addition, TIMP expression in crescent also increased by day 15 and more sustained increase until day 35. Mesangial matrix increased in glomeruli of anti-Thy-1 nephritic rats with a peak of day 8 and rapidly decreased to the normal level by day 30. The activities of MMPs in glomeruli of anti-Thy-1 nephritic rats showed little change during the experimental periods. Although TIMP expression in anti-Thy-1 nephritic rats increased from day 1 to day 8, then this expression decreased rapidly to the normal level by day 16. Trypsin-treated glomeruli of anti-GBM nephritic rats had a 12 fold increase in MMPs activities at day 5.
These results suggest that the maintained decline of MMPs activities in nephritic rats may be associated with the process of glomerulosclerosis.

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