G-CSFにて動員された顆粒球輸血の好中球機能

―G-CSF投与は正常ヒト好中球の遊走活性には無影響―

抄録

We attempted to investigate whether chemotaxis of neutrophils from healthy donors could be enhanced by G-CSF therapy. Two donors for granulocyte transfusions received intravenous administration of 2μg/kg G-CSF 16 hours before leukapheresis. Phagocytic activity of neutrophils from healthy donors after administration of G-CSF increased compared to those taken before G-CSF administration.
In contrast, random mobility and chemotaxis toward Zymosan-activated serum and filtrate of E. coli were unchanged. After G-CSF administration, neutrophils collected by leukapheresis showed augumented chemotaxis and random mobility as compared with those collected by venipuncture. However, leukapheresis procedure had no effect on phagocytosis. Neutrophil function was not affected by irradiation. Concerning the discrepancy between chemotaxis and phagocytosis, an increased neutrophil adhesion may result in depressive chemotaxis and enhanced phagocytosis.
And the fact that random mobility and chemotaxis of neutrophils enhanced after leukapheresis suggests that during the continuous flow centrifugation process, secondary cytokines may be released into circulation in response to G-CSF, which leads to the enhancement in neutrophil migration.