抄録
The use of immunoglobulin for human in vivo treatment has been limited to a great extent by the patient's immune response to non human antibodies in immunotherapy. Since it is hard to accepted the idea in public society that periodontal diseases cause death in humans, the development of safe antibodies in passive immunization is required. Several methods have been developed for the production of safe antibodies such as recombinant single chain Fv (ScFv) and human type antibodies. Porphyromonas gingivalis, a Gram-negative anaerobe, appears to be one of the most virulent microorganisms in relation to adult periodontitis. Coaggregation and hemagglutinin factors are important virulent factors in colonization on host tissues. This paper briefly introduces here the methods for preparation of safe immunoglobulins and summarizes our own attempts in preparations of ScFv and human type antibodies which are capable to inhibit the activities of coaggregation and hemagglutinin of P. gingivalis.
