Abstract
The effects of prostaglandins on human polymorphonuclear leukocytes (PMN) migration were studied in vitro by agarose plate method. None of the prostaglandins tested, including PGE1, E2, F2α and thromboxane B2 (TxB2) were chemotactic in concentrations ranging from 0.5 to 10 μg. However, pretreatment of PMN with PGE1 and PGF2α for 30 minutes at 0°C inhibited the chemotaxis induced by zymosan activated human serum as well as spontaneous migration of PMN, while pretreatment with TxB2 enhanced these reactions. These findings suggested that in some forms of inflammation, prostaglandins may play a controlling role in leukocytes migration. The inhibition or enhancement of PMN migration was not directly related to the changes of intracellular cyclic AMP levels by prostaglandins.
