Abstract
The effects of anti-inflammatory drugs on matrix degradation of articular chondrocytes of young rabbit were investigated by following methods; (1) release of 35SO4-labelled proteoglycans from cells and matrix layers into culture medium. (2) proteoglycanase and collagenase activities of the culture media of articular chondrocytes.
The anti-inflammatory drugs investigated were floctafenic acid, aspirin, dichlofenac Na, tiaprofenic acid, indomethacin and hydrocortisone. The results obtained were as follows; the decrease of proteoglycan release was observed with tiaprofenic acid, dichlofenac Na and indomethacin. The effects of anti-inflammatory drugs on proteoglycanase and collagenase activities were studied under the activated condition with interleukin 1. Hydrocorisone was found to inhibit these enzyme activities remarkably and NSAIDs except floctafenic acid and aspirin also inhibit these enzyme activities in moderate degree. From these results, some of anti-inflammatory drugs were recognized to inhibit degradating enzymes released from articular chondrocytes.
