Abstract
Renal function is modulated by glomerular mesangial cells which react to many substances. Arachidonic acid metabolites are important not only for the inflamation of glomeruli, but also for the regulation of renal function. We studied on arachidonic acid metabolism in the cultured rat mesangial cells.
Glomeruli isolated from 150g male Sprague-Dawley rats were treated with collagenase and trypsin and cultured in supplemented nutrient medium for 28 days. Experiments were perforemed on the first passage. Prostaglandins (PGsE) E2, F2α, D2 6-oxo-PGF1α, and Thromboxane B2 (TXB2) were measured by gas chromatography-masspectrometry (GC-MS) .
The mesangial cells produced PGE2>PGF2α>>PGD2, 6-oxo-PGF1α, and TXB2. Arginine vasopressin and angiotensin II stimulated PGE2 and PGF2α synthesis. High osmolaity condition by glucose or mannitol stimulated all PGs synthesis, especialy PGE2 and PGD2 when incubated with arachidonic acid.
These results suggest that mesangial PGE2 and PGD2 are important for the modulation of glomerular function.
