Abstract
The distribution and characteristics of renal prostaglandin E2 (PGE2) binding sites were examined in the normal and aminonucleoside-induced nephrotic rats by Scatchard analysis.
PGE2 binding site density in the medulla of normal rats was 2.7-fold higher than that in the cortex, but there was no significant difference in the dissociation constants (Kd) between cortex and medulla.
The Kd in the kidney medulla of the nephrotic rats were significantly higher (P<0.05) than that of normal rats, while the densities remained without changes.
These results indicate that PGE2 binding sites of the normal rat kidney are distributed in the medulla more than in the cortex, and the affinities of PGE2 binding sites in the nephrotic rat kidney are lower than those in normal rat kidney.
