Abstract
We have already reported an efficient method for simultaneous and quantitative determination of plasma PGs from bronchial asthmatic patients using reversed-phase HPLC. In the present study, the method was arranged and more sensitive determinations were obtained. Standard PGs were incubated with 9-anthryldiazomethane for one night, loaded on ODS column, and fluorescence was measured by the fluorometer. The addition of acetate into the eluate sharpened the peaks of TXB2 and 6-keto PGF1α. To separate residual interferences from PGs, samples were eluted with 75% (v/v) methanol/25% (v/v) water in the presence of 1.5% (w/v) acetate. The quantitation of 10 pg PGE1, PGE2, PGF2α, 20pg 6-keto PGF1α, 50pg TXB2, and 60pg PGA2 became possible with these methods.
