免疫調節剤Lobenzarit・2Na (CCA) の細胞骨格系への作用

―2. LPS刺激されたMφの細胞骨格系の応答に対する作用―

抄録

We reported in a preceding paper that Lobenzarit-2Na (CCA) affected RNA polymerase-I activity in nuclear suspension prepared from rat hepatocytes. This effect of CCA on RNA polymerase-I was thought to be the secondary effect, that is, CCA primarily modified the reactivity of cytoskeletal components around the nuclei.
Previously it was also reported that CCA inhibited the IL-1 production by LPS-stimulated macrophages. So we studied whether CCA could modify the cytoskeletal re-organization process in LPS-stimulated macrophages or not.
Results reported here show that microfilament system in macrophages changes its configulation in a biphasic manner within 10 min after LPS stimulation. CCA reduced the magnitude of this configurational change without affecting the timing of process.
Because CCA could be transported into macrophages through some kind of carrier in plasma membrane, this effect of CCA on microfilament system was likely to be the direct action in cytoplasm.