Age-Associated Reduction of Sinus Macrophages in Human Mesenteric                    Lymph Nodes

Kosuke Kanemitsu; Rin Yamada; Cheng Pan; Hirotake Tsukamoto; Hiromu Yano; Takuya Shiota; Yukio Fujiwara; Yuji Miyamoto; Yoshiki Mikami; Hideo Baba; Yoshihiro Komohara

doi:10.3960/jslrt.24001

Age-Associated Reduction of Sinus Macrophages in Human Mesenteric Lymph Nodes

Kosuke Kanemitsu, Rin Yamada, Cheng Pan, Hirotake Tsukamoto, Hiromu Yano, Takuya Shiota, Yukio Fujiwara, Yuji Miyamoto, Yoshiki Mikami, Hideo Baba, Yoshihiro Komohara

Author information

Kosuke Kanemitsu
Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan,
Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Honjo 860-8556, Kumamoto, Japan,
Rin Yamada
Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan,
Department of Diagnostic Pathology, Kumamoto University Hospital, Kumamoto, Japan,
Cheng Pan
Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan,
Hirotake Tsukamoto
Division of Clinical Immunology and Cancer Immunotherapy, Center for Cancer Immunotherapy and Immunobiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan,
Hiromu Yano
Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan,
Takuya Shiota
Department of Diagnostic Pathology, Kumamoto University Hospital, Kumamoto, Japan,
Yukio Fujiwara
Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan,
Yuji Miyamoto
Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Honjo 860-8556, Kumamoto, Japan,
Yoshiki Mikami
Department of Diagnostic Pathology, Kumamoto University Hospital, Kumamoto, Japan,
Hideo Baba
Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Honjo 860-8556, Kumamoto, Japan,
Yoshihiro Komohara Corresponding author
Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan,
Center for Metabolic Regulation of Healthy Aging, Kumamoto University, Kumamoto, Japan

Keywords: Colorectal cancer, lymph node, sinus macrophage

JOURNAL OPEN ACCESS Advance online publication

Article ID: 24001

DOI https://doi.org/10.3960/jslrt.24001

Details

Abstract

There are numerous macrophages and dendritic cells in lymph nodes (LNs). Recent studies have highlighted that sinus macrophages (SMs) in LNs possess antigen-presenting capabilities and are related to anti-cancer immune responses. In this study, we assessed the distribution of SMs in mesenteric LNs removed during surgery for colorectal cancer. A marked reduction of SMs was noted in elderly patients, particularly those over 80 years old. We observed a disappearance of CD169-positive cells in LNs where SMs were reduced. In silico analysis of publicly available single-cell RNA sequencing data from LNs revealed that CD169-positive macrophages express numerous genes associated with antigen presentation and lymphocyte proliferation, similar to dendritic cells' functions. In conclusion, our study demonstrates that SMs, potentially crucial for immune activation, diminish in the LNs of elderly patients. This reduction of SMs may contribute to the immune dysfunction observed in the elderly.

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