Abstract
A human monocytic leukemia cell line, NOMO-1, established from a female with acute monocytic leukemia (M5a), was analyzed for the presence of the activity of tissue factor, plasminogen activator (PA) and plasminogen activator inhibitor (PAI). NOMO-1 has promonocytic characteristics, and differentiation into macrophage-like cells was induced by 12-o-tetradecanoyl phorbol-13-acetate (TPA, 10-7M). After the addition of TPA, fibronectin was demonstrated in NOMO-1 cells, and Interleukin-1 (IL-1) α and IL-1β increased in the culture medium. TPA enhanced the secretion of tissue factor from NOMO-1 cells, while warfarin (1ng/ml) inhibited the secretion PA activity was gradually increased during the 17 culture days. Fibrin autography demonstrated that PA secreted from NOMO-1 cells is urokinase type and has M.W. of 55, 000. TPA, lipid A (20μg/ml) and Vitamin-D (1-25- (OH)2D3, 10-8M) profoundly enhanced PA activity immediately after the addition of these chemicals. PAI was gradually increased during 17 culture days, and the addition of TPA, lipid A and Vit-D strikingly enhanced the secretion of PAI. Reverse fibrin autography revealed M.W. of 47, 000. NOMO-1 cells may be useful model for the study of human monocytic coagulation and fibrinolysis system.
