Localizations of lactoferrin and lysozyme in the peripheral blood cells and bone marrow cells obtained from normal volunteers were investigated by an electron microscopic immunogold double stainings. Both lactoferrin and lysozyme were present in the same granules of neutrophils. Mast cell granules and some phagolysosomes of bone marrow reticulum cells also showed similar double stainabilities. In contrast, monocyte granules and eosinophil granules contained exclusively lysozyme. Neither lactoferrin nor lysozyme was observed in blastoid cells, megakaryocytes, platelets, erythroblasts, red blood cells, plasma cells, lymphocytes and basophils.
The electron microscopic immunogold double stainings are useful for detecting a couple of antigens on a single cell.
