2008 Volume 29 Issue 2 Pages 164-168
Japanese association of 5-aminolevulinic acid (5-ALA: porphyrin precussors) was started at 2001 in Okazaki first meeting. The third meeting was combined with the 9th world congress of the international photodynamic association (9th IPA) as the satellite small first international symposium of the 5-ALA at 2003 in Fukuoka, Japan.
The studies of photodetection (PD) and photodynamic therapy (PDT) of clinical cancer in Japan were mainly doing of PD for glioblastoma (GBM) as the fluorescent guide of the cancer tissue during the operation in neurosurgery and studies of PDT for skin cancers and acne in dermatology areas also has being done using the 5-ALA. Recently, the PD of bladder cancer and stomach cancer are applied during the operation using the endoscopes in Japan.
In basic research using a cultivated cancer cell line and the transplanted tumor model, we applied the many kinds of the 5 ester derivatives (high purity-99.99% produces from a Japanese company) of the 5-ALA for a cultivated human leukemia cell line (HL-60) to find the protoporphyrin-IX (Pp-IX) uptake in the cell and to find the photodynamic cell damages. Our Japanese group developed the fluorescent analyzer system using a blue-laser (405nm) for the clinical and animal study. Furthermore, it was also developed for the new laser of 2 wavelengths 635 (for Pp-IX) and 670 nm which is targeting of the photoproduct of protoporphyrin-IX (Pp-IX). It was reviewed the photoproduct effect as a new photosensitizer in human leukemia cells using the laser..
