Abstract
We compared the Phototoxicity effect of the cancer cells and normal cells by PDT (photodynamic therapy) used a laser diode as an optical source. It was investigated the accumulation of Talaporfin in cancer cells after incubation in solution and confirmed Talaporfin can kill cancer cells selectively by photo-irradiation. In the experiment, the WFB (rat fetus fibroblast) as normal cells and W31 as cancer cells were used. W31 is the malignant transformed cell from WFB. Fluorescence image was measured from cancer cells incubated in Talaporfin solution with a fluorescence microscope. It was found that the fluorescence peak of Talaporfin in cell has red-shifted and the lifetime which measured by streak-camera was slightly increased as compared to Telaporfin solution. The reason is the Talaporfin combined the bio-molecules in the cancer cell and the emission situation is changed. The measurement of the optical absorbance of Telaporfin in the cell, it confirmed that the Talaporfin could be accumulated in cancer cells more than in normal cells. Furthermore, the viability of the cells was assessed by use Trypan Blue Stain after PDT. It was clarified that Talaporfin could be accumulated in cancer cells more than in normal cells and lead to selective cancer cell death.
