Dedvelopement of Real-Time CARS Microscope and Application to Living Cell Measurement

Abstract

Raman microscopy visualizes molecular spices without any staining, because molecular vibrations that all molecules have are sensitive to molecular spices. We developed a real-time CARS (coherent anti-Stokes Raman scattering, which is one of nonlinear Raman scattering) microscopy system. We demonstrate three dimensional lipid distribution in a cell, laser-induced disruption of a lipid rich organelle, and plasma membrane repairing of disrupted plasma membrane.