Photoacoustic Microscopy with Nonlinear Optical Effects to Visualize Deep Structures with High Spatial Resolution

Abstract

Recently, photoacoustic microscopy(PAM)has attracted attention to visualize deep structures in living tissues. However, it is difficult to improve the spatial resolution of PAM without using high-frequency components of photoacoustic waves, which are not suitable for deep imaging. To overcome this drawback, we developed two-photon absorption-induced photoacoustic microscopy(TP-PAM). The spatial resolution in TP-PAM is determined by two-photon absorption(TPA). The use of low-frequency ultrasonic components generated by TPA enables PAM to visualize deeper structures while preserving the high spatial resolution.