Laser Assisted Hatching

Abstract

In the embryos made by assisted reproductive technology (ART), the hatching has been frequently inhibited by the hardening of the zona pellucida due to the in vitro culture. Assisted hatching (AH) is a technique to promote the hatching of embryo by breaching a part of zona pellucida using mechanical or chemical method. Laser technologies became increasingly common for zona manipulation. Laser assisted hatching (LAH) is an important technique to support the ART.