Photodynamic effects of gold vapor laser on cultured hepatocellular carcinoma cells (JTC-16)

-A compared study between argon-dye laser and gold vapor laser-

Abstract

A pulsed gold vapor laser (627.8nm) was experi-mentary compared with a continuous wave argon-dye laser (630nm) in terms of photodynamic effects on cultured hepatocellular carcinoma cells (JTC-16). The photodynamic effects on the cells were evaluated by a phase contrast microscope and erythrosine B exclusion test at 24 hours after laser irradiation. The porphyrin examined was hematoporphyrin.
Gold vapor laser was more effective than argon-dye laser on the cells, when the concentration of hematoporphyrin was 15μg/ml. Photodynamic effects of laser would be due to photochemical response, since laser irradiation did not elevate the temperature of culture medium.
This result support the superiority of gold vapor laser in photodynamic therapy