Abstract
The effect of photodynamic therapy (PDT) using argon-pumped dye laser on new photosensitizer, PH-1126 (amine in the d-ring of pheophorbide, Hamari Chemical Co. Ltd. Osaka) on T9 glioma, was investigated.
T9 cells, in vitro, were incubated independently with HpD and PH-1126, and irradiated. Using 1μg/ml of PH-1126, 100% cell death needed for 34J/cm2. PDT with PH-1126 was more favorable than that with HpD in tumorcidal effect.
Rat brain tumor, in vivo, was established by implanting T9 cells in Fischer 344 male rat. PDT was carried out 24hours after administration of 10mg/kg PH-1126 with craniotomy, in which the tip of an optical fiber was contact with the tumor surface. Histologically, the depth of tumor necrosis was 3.7mm at 30joules, 5.3mm at 60joules, and 6.4mm at 120joules.
It is suggested that PH-1126 is one of useful photosensitizers for treatment of brain tumors.
