Abstract
We studied the change of tumor blood volume at PDT using the reflectance spectrophotometer. The tumor (GCA to be established by serial implantation in our institute; adenocarcinoma papillo-tubulare) was implanted in the back of nude-mouse. When tumors became 1cm in diameter, HPD (10mg/kg or 50mg/kg) was injected intraperitoneally and then 48 horus after injection GVL or ADL of intense of 280 mW/cm2 was irradiated for three minutes.
The change of blood volume of tumors was determind by IHB and ISO using reflectance spectrophotometer, before and after laser irradiation.
At GVL was irradiated, increase of IHB and decrease of ISO were recognized. On the other hand when ADL was irradiated, the similar change of IHB and ISO was recognized but the degree of the change was less than that of GVL irradiation.
It is revealed by this study that the decrease of blood volume of tumors occurred in PDT. And this is assumed as one of the mechanism of PDT. The degree of change at GVL irradiation was superior than that of ADL irradiation, and then it is considered as one of the reason that GVL is excellent compared with ADL in PDT.
