Abstract
To investigate the mechanism of cell photostimulation by low-intensity laser irradiation, we studied the influences of different wavelength irradiations (351nm, 465nm, 514nm) of Ar laser on the mitochondrial respiration.
Isolated ginea-pig liver mitochondria and isotonic buffer (0.25M saccharose, 2mM MgCl2, 10mM K2HPO4, 10mM KCl, 0.2mM EDTA, 4mM HEPES, pH 7.4) were introduced into a fixed temperature reaction chamber (37°C, 2ml). Either in respiratory condition of state 4 (addition of 4mM saccinate into the chamber) or state 3 (addition of 4mM saccinate plus 180μM ADP into the chamber), the oxygen consumption (O2 cons.) in the chamber was measured using a oxygen electrode with or without laser irradiation.
In state 4, shorter wavelength (351nm, 465nm) laser irradiation increased the O2 cons. In contrast, in state 3, only 514nm laser irradiation increased the O2 cons..
These studies suggest that the Ultra-Violet laser irradiation may injure the mitochondrial inner-membrane, while the green laser irradiation may promote the ability of ATP-synthesis.
