Abstract
A new instrument of Laser Flowgraphy is developed to visualize microcirculation map under microscopy. An object tissue, a web of Xenopus, is illuminated by an Argon laser spot, magnified through one optical path of the stereo microscope and imaged onto an area sensor, where the motion of the speckle field result from the blood flow in the periphery is detected. The output signal is processed by the standard Laser Flowgraphy technique and the results of the 2-D blood flow map are displayed in color graphics. Two different measures of blood flow, 1) Average Difference (AD) of successive frame pairs and 2) average BLur (BL) in a series of frames are evaluated and displayed simultaneously in two windows. Comparing these two measures, AD and BL maps, more comprehensive analyses have become possible for the blood circulation in the tissue under microscopy.
