1993 Volume 14 Issue Supplement Pages 321-324
It is necessity to have experimental animal models which have a tumor on the bronchus for the studies of photodynamic diagnosis and therapy of lung cancer. However, it is a quite hard work to devepole the malignant tumor in the animal bronchus experimentally. The VX-7 cell line, rabbit squamous cell carcinoma, is relatively easy to subculture and generally used in many studies. The aim of this study is to establish the methods how to develop the bronchogenic tumor in the rabbits using newly-developed ultra-thin fiberscope and to performe the photodiagnosis with this model. 15 Japanese white male rabbits weighing 3.5kg weight were used in this study. Under the general anesthesia with 50mg/kg intravenous pentobarbital, intubation was performed with 3.5mm cystoscope for infant. Inoculation of VX-7 tumor using TBAC needle under ultra-thin fiberscope. Fiberscopy was performed once a week. Three weeks after cancer cell transplantation, Photofrin was injected intravenously and fluorescence was examined with a 405 nm laser beam using an excimer dye laser fluorescence-image analyzer. All rabbits were sacrificed at 4 week after inoculation and performed histopathological examination. The fluorescence spectrum of Photofrin with its characteristic maximum peaks at 630nm and 670nm were obtained only in cancer lesions, while surrounding normal tissue demonstrated negligible uptake of dye. It is suggested that this model cancer of carina has been useful in photodynamic diagnosis, photodynamic therapy and their basic study.
