1993 Volume 14 Issue Supplement Pages 337-340
HAT-D01 is a typical fluorescent diagnostic agent without phototoxicity because the exited triplet status is extremely short and the molecular structure has meta-phenylene spacer-bearing porphyrin hetero-dimers.
HAT-D01 in PBS was administered intravenously to tumor bearing Balb/C mice via the dorsal tail vein at doses of 80mg/kg body weight. The following tissues were removed: brain, lungs, heart, liver, spleen, intestine, kidneys, skin and tumors at 0.5, 1, 4 & 24 hours after drug administration using 3 mice per time point. Some points of various normal tissues and tumor were chosen and were examined by a new excimer pulse dye laser diagnostic system (Hamamatsu Photonics K. K., Hamamatsu, Japan) using a fiberscope to perform a time-series study of the uptake of HAT-D01 in the various organs and tumors.
The relative intensity of HAT-D01 in the various organs became optimal around 0.5 to 1 hours after i. v. injection at a dose of 80mg/kg. When the relative intensity of HAT-D01 from the tumor was set at 100%, at 0.5 hours after i. v. injection the intensity in other organs was as follows: skin 96%, intestine 52% and other tissues (brain, lungs, heart, liver, spleen and kidneys) below 1%. Therefore, the HAT-D01 in normal tissues except skin decreased gradually with time for up to 24 hours.
These results suggest that HAT-D01 may be useful for clinical photodynamic diagnosis (PDD) of tumors.
