Abstract
Recently low power laser has been used widely in clinicas. The low power semiconductor laser irradiation shows wound healing and analgeic effects at the damagedsite.
However, the mechanism of these semiconductor laser effects has not yet been elucidated. To investigate the influence of semiconductor laser irradiation on cultured human keratinocytes, we studied the cell vialility using MTT assay and measured 1L-1α by ELISA. The number of viable cells was significantly increased by semiconductor laser irradiation for 15sec (115±0.5%of control) and 20sec (112±1.0%). The production of 1L-1α was also increased by laser irradiation for 20sec (increase of 33±5.0pg/ml). These results seggest that low power laser displays significant biological effects on human kerationcytes for activation.
