Abstract
Confocal laser scanning microscopy (CLSM) has been used in various fields of medical and biological sciences. In this article, applications of CLSM for the biological transport study in the biological cells and tissues were reviewed, mainly from the authors' recent studies. At first, the basic idea of the CLSM was briefly described, and then dye selection for the physiological study was summarized. For the physiological applications, we mentioned about two case-studies: 1) the macromolecules transport into endothelial cells, and 2) Ca2+ mobilization in the median giant fiber of an earthworm. At last, future applications of the CLSM for the biological transport were also indicated.
