Abstract
We studied the distribution and the photocytotoxicity of photosensitizers in the cultured tumor cells. A cultured Colon 26 cell line derived from BALB/c was used. The hydrophilic photosensitizers, ATX-S10Na (II), PAD-S31 and the amphipathic photosensitizer, Photofrin® were used. The cellular localization and the cell-damage mechanisms of the photosensitizers were analyzed under a video-microscope.
The localization of ATX-S10Na (II) and PAD-S31 was mainly in lysosomes of the cultured cells, that was relocalized in the cytoplasm within a second of illumination. The localization of Photofrin® was in the cytoplasm of the cultured cells. The cells containing each photosensitizer were swollen and shrunken and died by exposure of the light.
