Abstract
Using spectroscopic, tissue culture and biochemical analysis, HPLC, and electromicroscopic techniques and so on, basic investigations of photodynamic therapy (PDT) using hematoporpyrin derivative (HpD) have been performed. HpD may make an aggregated form after cellular incorporation which bound to mainly mitochondria and cell membrane. The aggregated HpD exerted strong photodynamic reactions and produced the damage of protein synthesis followed by DNA synthesis disturbance. The PDT with argon-dye laser (ADL), 630nm, was performed on superficial tumors of the penis and bladder. Less than 1.5cm tumors in size achieved to CR using the irradiation of output 200-300mW/cm2 via a 400um quartz fiber for more than 10min. Integral whole bladder wall PDT was used for CIS of the bladder with the light dose of 10-20J/cm2 and CR was obtained in most cases. A gold vapor laser (GVL) was studied in terms of tissue penetration and tumor destruction. The results obtained supported the superiority of GVL over ADL in enhancing PDT effect and in introducing 43°C hyperthermia.
