Abstract
In the previous works, it was made clear that extremely close relationship exists between Parotin and calcium metabolism. Therefore, the author investigated the influence of Parotin on the chick embryo added with calcium chloride, thereby he attempted to trace the correlationship between calcium and Parotin from the pharmacological view point in the embryonal stage. As was done in the previous works, determination of the general development and the bone as well as the histological examination of bone were conducted for the present work.
One injection dose for 1 egg was 0.1 cc of 37.73% solution of calcium chloride containing the requisite amount of Parotin. Consequently, the amount of calcium chloride given to each egg was 37.73 mg. The amounts of Parotin given to each egg were 0.2 mg, 0.01 mg, 0.1γ and 0.001γ. Untreated eggs and those to which calcium chloride solution only was injected were simultaneously incubated as controls.
The eggs were divided into 3 groups, and each of them were taken out after 7 days', 14 days' and 18 days' incubation respectively, then the embryos were taken out and weighed, and the growth of feathers were observed, and the measurements of the femur were taken. Thereafter, they were fixed with 1: 10 solution of formalin.
As the results of the above examinations, the following conclusion was drawn:
The hindrance to the development to chick embryo caused by the addition of calcium chloride is almost completely removed by the addition of the salivary gland hormon, Parotin.
