心臓神経から遊離されるChemical Mediatorの定量法について

抄録

Employing the heart nerve heart preparations of Bufo vulgaris, the authors gave repetitive stimuli of varying frequency to the vagal nerve or to the sympathetic nerve for 15 seconds and calculated the effect thereof on the pulse curve to draw the frequency action curves. Further, the authors investigated the relationships between the concentration of acethylcholine or of adrenaline and their effect by irrigating with these chemicals in order to draw the concentration action curves. In view of the fact that these curves satisfies the theoretical formula by Rosenbueth, by over-lapping these two curves, the authors attempted to find out the frequency of the stimulus corresponding to a certain concentration in percentage of acethylcholine or of adrenaline through irrigation.
Further, the authors calculated the amount of acethylcholine or of adrenaline in the irrigation fluid consumed by the cardiac muscle by examining the out-flowing fluid, and successfully estimated, from the above frequency-concentration relationship, the amount of acethylcholine or of adrenaline-like substance freed from the end of the vagal nerve and the sympathetic nerve respectively after one single maximal stimulation.