Journal of The Showa Medical Association
Online ISSN : 2185-0976
Print ISSN : 0037-4342
ISSN-L : 0037-4342
STUDIES ON THE PHAGOCYTIC POWER OF NEUTROPHIL LEUCOCYTES AND PITUITARY-ADRENOCORTICAL FUNCTION
Part 1. A Simplified Method measuring the Phagocytic Power of Neutrophil Leucocytes
Iwao Honda
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1957 Volume 17 Issue 4 Pages 393-396

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Abstract
In the present paper, the author tried out a simplified method to measure the phagocytic power of leucocytes employing a drop of blood, and made an investigation on the properties of the phagocytosis.
Method: A drop of blood, which contains heparin to prevent coagulation, is mixed with carbon particle suspension on a concave slide glass and the mixture is incubated at 370°C. Smear preparations are made from it for examinations.
Results:
1. The preparations made according to this method was neat and the carcon particles were readily differentiated.
2. The degree of the phagocytosis was influenced by the length of incubation period and the number of carbon particles mixed.
3. The rate of phagocytosis was hardly influenced by the length of incubation period and the number of carbon particles mixed.
4. No difference in the results was recognized between the present method and other methods which employ a relatively larger amount of blood.
In view of the above findings, the author considered that the present method is a utilizable one with a minute amount of blood, and that the degree of phagocytosis and the rate of phagocytosis are essentially different matters.
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